Molecular Cloning: A Laboratory Manual, Book 3 |
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Page B-23
... Electrophoresis Buffers Tris - phosphate ( TPE ) Working solution Buffer Tris - acetate ( TAE ) 1X : 0.04 M Tris ... ( electrophoresis grade ) ( pH 8.3 ) 50 ml 10 % SDS ( electrophoresis grade ) " A precipitate forms when concentrated ...
... Electrophoresis Buffers Tris - phosphate ( TPE ) Working solution Buffer Tris - acetate ( TAE ) 1X : 0.04 M Tris ... ( electrophoresis grade ) ( pH 8.3 ) 50 ml 10 % SDS ( electrophoresis grade ) " A precipitate forms when concentrated ...
Page 1-1
... electrophoresis alkaline analysis of first- and second - strand cDNA synthesis , 8.5 analysis of single - stranded DNA , 6.20-6.21 bromocresol green as tracking dye in electrophoresis , 6.12 , 6.21 gel - loading buffer , 6.12 , B.24 ...
... electrophoresis alkaline analysis of first- and second - strand cDNA synthesis , 8.5 analysis of single - stranded DNA , 6.20-6.21 bromocresol green as tracking dye in electrophoresis , 6.12 , 6.21 gel - loading buffer , 6.12 , B.24 ...
Page 1-16
... electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning vectors , 4.2 , 4.7 problems encountered in use ...
... electrophoresis FIGE ( field - inversion gel electrophoresis ) . See Pulsed - field gel electrophoresis Filamentous bacteriophages . See also M13 bacteriophage ; Phagemids as cloning vectors , 4.2 , 4.7 problems encountered in use ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml