Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 76
Page 17-22
... enzyme leaves a 5 ' overhang upon digestion . Site 2 must lie between site 1 and the start of the gene , and it must be closer to the start of the gene than to site 1 . 2. Cleave 5 μg of the DNA at site 2 with the appropriate ...
... enzyme leaves a 5 ' overhang upon digestion . Site 2 must lie between site 1 and the start of the gene , and it must be closer to the start of the gene than to site 1 . 2. Cleave 5 μg of the DNA at site 2 with the appropriate ...
Page F-10
... enzyme buffer appropriate restriction enzyme 70 μl 10 με 20-50 units Mix , and then incubate the reaction for 4 hours at the optimal tempera- ture for the restriction enzyme . 4. Add an additional 10 units of the restriction enzyme ...
... enzyme buffer appropriate restriction enzyme 70 μl 10 με 20-50 units Mix , and then incubate the reaction for 4 hours at the optimal tempera- ture for the restriction enzyme . 4. Add an additional 10 units of the restriction enzyme ...
Page F-11
... enzyme along with the large - scale reaction . Store the small - scale reaction without the enzyme at 4 ° C . e . Analyze both small - scale reactions on a 1 % agarose gel . If the reaction worked well , the plasmid DNA should be ...
... enzyme along with the large - scale reaction . Store the small - scale reaction without the enzyme at 4 ° C . e . Analyze both small - scale reactions on a 1 % agarose gel . If the reaction worked well , the plasmid DNA should be ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml