Molecular Cloning: A Laboratory Manual, Book 3 |
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Page vi
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
... Ethidium Bromide Gradients CONTINUOUS GRADIENTS 1.42 DISCONTINUOUS GRADIENTS 1.44 1.42 Removal of Ethidium Bromide from DNAs Purified by Equilibrium Centrifugation in CsCl - Ethidium Bromide Gradients 1.46 METHOD 1 : EXTRACTION WITH ...
Page E-5
... ethidium bromide . Spectrophotometric Determination of the Amount of DNA or RNA For quantitating the amount of DNA or RNA , readings should be taken at wavelengths of 260 nm and 280 nm . The reading at 260 nm allows calculation of the ...
... ethidium bromide . Spectrophotometric Determination of the Amount of DNA or RNA For quantitating the amount of DNA or RNA , readings should be taken at wavelengths of 260 nm and 280 nm . The reading at 260 nm allows calculation of the ...
Page E-8
A Laboratory Manual Joseph Sambrook, Tom Maniatis. DECONTAMINATION OF ETHIDIUM BROMIDE SOLUTIONS Caution : Ethidium bromide is a powerful mutagen and is moderately toxic . Gloves should be worn when working with solutions that contain ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. DECONTAMINATION OF ETHIDIUM BROMIDE SOLUTIONS Caution : Ethidium bromide is a powerful mutagen and is moderately toxic . Gloves should be worn when working with solutions that contain ...
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Common terms and phrases
acetate acrylamide Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract final concentration fragment gene H H H H OH hybridization Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl NH3 H nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml