Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 52
Page ix
... EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING EXTRACTS FROM ONE LYSOGEN 2.105 PROTOCOL II : PACKAGING IN VITRO USING ONE EXTRACT 2.107 Identification and ...
... EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING EXTRACTS FROM ONE LYSOGEN 2.105 PROTOCOL II : PACKAGING IN VITRO USING ONE EXTRACT 2.107 Identification and ...
Page 16-58
... extracts . This is best achieved by cotransfecting the cells with two plasmids - one that carries the construct under investigation and another that constitutively expresses an activity that can be assayed in the same cell extracts ...
... extracts . This is best achieved by cotransfecting the cells with two plasmids - one that carries the construct under investigation and another that constitutively expresses an activity that can be assayed in the same cell extracts ...
Page 16-66
... extracts are prepared as described on pages 16.59 and 16.60 , steps 1-3 ( except reserve 30 μl of the extract for the B - galactosidase assay ) . The extracts that are to be used for assay of CAT activity should be heated to 65 ° C for ...
... extracts are prepared as described on pages 16.59 and 16.60 , steps 1-3 ( except reserve 30 μl of the extract for the B - galactosidase assay ) . The extracts that are to be used for assay of CAT activity should be heated to 65 ° C for ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml