Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xviii
... Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO NITROCELLULOSE FILTERS 9.41 Transfer of DNA from Agarose Gels to Nylon Membranes 9.42 CAPILLARY TRANSFER ...
... Filters 9.38 CAPILLARY TRANSFER OF DNA TO NITROCELLULOSE FILTERS 9.38 SIMULTANEOUS TRANSFER OF DNA FROM A SINGLE AGAROSE GEL TO TWO NITROCELLULOSE FILTERS 9.41 Transfer of DNA from Agarose Gels to Nylon Membranes 9.42 CAPILLARY TRANSFER ...
Page E-18
... filters ( 2.4 - cm diameter ) . Impale each of the filters on a pin stuck into a polystyrene support . 2. Spot an accurately known volume ( up to 5 μl ) of the sample to be assayed on the center of each of two labeled filters . One of ...
... filters ( 2.4 - cm diameter ) . Impale each of the filters on a pin stuck into a polystyrene support . 2. Spot an accurately known volume ( up to 5 μl ) of the sample to be assayed on the center of each of two labeled filters . One of ...
Page E-19
... filter cpm in unwashed filter = proportion incorporated proportion incorporated × total wt . = total amt . of product cpm incorporated total amt . of product Adsorption to DE - 81 Filters = specific activity DE - 81 filters are ...
... filter cpm in unwashed filter = proportion incorporated proportion incorporated × total wt . = total amt . of product cpm incorporated total amt . of product Adsorption to DE - 81 Filters = specific activity DE - 81 filters are ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml