Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 30
Page 16-49
... final concentration of 250 μg / ml . Continue incubation overnight . See Chapter 1 , pages 1.21 and 1.33 , for a discussion of the use of chloramphenicol in the amplification of plasmids . 2. Recover the bacterial cells from 50 ml of ...
... final concentration of 250 μg / ml . Continue incubation overnight . See Chapter 1 , pages 1.21 and 1.33 , for a discussion of the use of chloramphenicol in the amplification of plasmids . 2. Recover the bacterial cells from 50 ml of ...
Page 18-45
... final concentration of 0.5 M. For the second wash , standard NET - gel buffer is supplemented with SDS ( final concentration 0.1 % ) . The third wash is carried out with 10 mM Tris · Cl ( pH 7.5 ) , 0.1 % NP - 40 . • RIPA buffer ( see ...
... final concentration of 0.5 M. For the second wash , standard NET - gel buffer is supplemented with SDS ( final concentration 0.1 % ) . The third wash is carried out with 10 mM Tris · Cl ( pH 7.5 ) , 0.1 % NP - 40 . • RIPA buffer ( see ...
Page E-10
... final concentration of 0.01 M. 2 TABLE E.1 Salt Solutions Stock solution ( M ) Final E.10 Commonly Used Techniques in Molecular Cloning Precipitation with Ethanol or Isopropanol E 10.
... final concentration of 0.01 M. 2 TABLE E.1 Salt Solutions Stock solution ( M ) Final E.10 Commonly Used Techniques in Molecular Cloning Precipitation with Ethanol or Isopropanol E 10.
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml