Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-26
... foreign protein . EPSTEIN - BARR VIRUS VECTORS The human herpesvirus , Epstein - Barr virus ( EBV ) , transforms resting human B lymphocytes into dividing blast cells that can proliferate indefinite- ly in culture ( for review , see ...
... foreign protein . EPSTEIN - BARR VIRUS VECTORS The human herpesvirus , Epstein - Barr virus ( EBV ) , transforms resting human B lymphocytes into dividing blast cells that can proliferate indefinite- ly in culture ( for review , see ...
Page 16-28
... foreign gene and express moderate amounts of the protein of interest . Larger quantities of foreign protein can be obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign ...
... foreign gene and express moderate amounts of the protein of interest . Larger quantities of foreign protein can be obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign ...
Page 17-31
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression of Secreted Foreign Proteins Secretion of the foreign protein is an alternative to intracellular expression . This is accomplished by fusing the coding sequence to DNA ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Expression of Secreted Foreign Proteins Secretion of the foreign protein is an alternative to intracellular expression . This is accomplished by fusing the coding sequence to DNA ...
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Common terms and phrases
acetate acrylamide activity Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA Biol blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted equal volume Escherichia coli ethanol ethidium bromide expression extract final concentration gene H H OH Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel Sephadex sequences single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml