Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-6
... Four to 6 weeks later , inject 0.25 ml of freshly prepared emulsion into another limb . Limbs should be used in rotation every 4-6 weeks for subsequent booster injections . • For intradermal injection : Inject 0.025 ml of emulsified ...
... Four to 6 weeks later , inject 0.25 ml of freshly prepared emulsion into another limb . Limbs should be used in rotation every 4-6 weeks for subsequent booster injections . • For intradermal injection : Inject 0.025 ml of emulsified ...
Page F-1
... four ways that such conversion is commonly accomplished : • Partial filling of incompatible recessed 3 ' termini with the Klenow fragment of E. coli DNA polymerase I ( Zabarovsky and Allikmets 1986 ) . As discussed in Chapter 5 , this ...
... four ways that such conversion is commonly accomplished : • Partial filling of incompatible recessed 3 ' termini with the Klenow fragment of E. coli DNA polymerase I ( Zabarovsky and Allikmets 1986 ) . As discussed in Chapter 5 , this ...
Page F-2
... four dNTPs are added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) whether partial or complete filling is required . For example , to fill recessed 3 ' termini created ...
... four dNTPs are added to the reaction depends on ( 1 ) the sequence of the protuding 5 ' termini at the end ( s ) of the DNA and ( 2 ) whether partial or complete filling is required . For example , to fill recessed 3 ' termini created ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml