Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-25
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
... fragment prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described ...
Page F-2
... fragment of E. coli DNA polymerase I GAATTOH 3 ' CTTAAp5 ' On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : 3 ' dATP dTTP dCTP AAGCTOH TTCGAр5 ' 3 ' 4. Inactivate the Klenow fragment of E ...
... fragment of E. coli DNA polymerase I GAATTOH 3 ' CTTAAp5 ' On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : 3 ' dATP dTTP dCTP AAGCTOH TTCGAр5 ' 3 ' 4. Inactivate the Klenow fragment of E ...
Page F-2
... fragment of E. coli DNA polymerase I On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : Аон · AOH 3 . 3 ' TTCGAр5 ' dATP dTTP dCTP dGTP Klenow fragment of E. coli DNA polymerase I AAGCTOH 31 3 ...
... fragment of E. coli DNA polymerase I On the other hand , all four dNTPs are required to fill recessed termini created by HindIII : Аон · AOH 3 . 3 ' TTCGAр5 ' dATP dTTP dCTP dGTP Klenow fragment of E. coli DNA polymerase I AAGCTOH 31 3 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml