Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-3
... fusion protein is usually made at high levels because transcription and translation initiation are directed by normal E. coli sequences . • Fusion of foreign sequences to E. coli genes often results in products that are more stable than ...
... fusion protein is usually made at high levels because transcription and translation initiation are directed by normal E. coli sequences . • Fusion of foreign sequences to E. coli genes often results in products that are more stable than ...
Page 17-4
... Fusion Genes Several vector systems have been developed for the expression of lacZ fusion genes . One such system , the pUR series of vectors ( Rüther and Müller - Hill 1983 ) , has cloning sites for BamHI , SalI , PstI , XbaI , HindIII ...
... Fusion Genes Several vector systems have been developed for the expression of lacZ fusion genes . One such system , the pUR series of vectors ( Rüther and Müller - Hill 1983 ) , has cloning sites for BamHI , SalI , PstI , XbaI , HindIII ...
Page 17-9
... fusion protein should appear as a novel band migrating more slowly than the intense B - galactosidase band in the control . It is not uncommon for a protein ... Fusion Proteins Preparation of Fusion Proteins for Antibody Production 17 8 7.
... fusion protein should appear as a novel band migrating more slowly than the intense B - galactosidase band in the control . It is not uncommon for a protein ... Fusion Proteins Preparation of Fusion Proteins for Antibody Production 17 8 7.
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml