Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-28
... gene and express moderate amounts of the protein of interest . Larger quantities of foreign protein can be obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign gene is ...
... gene and express moderate amounts of the protein of interest . Larger quantities of foreign protein can be obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign gene is ...
Page 16-71
... gene . The above three examples illustrate the type of selection or screening that is essential for the initial identification of cells that have taken up and express the desired gene . Several strategies have been used to actually ...
... gene . The above three examples illustrate the type of selection or screening that is essential for the initial identification of cells that have taken up and express the desired gene . Several strategies have been used to actually ...
Page 17-15
... gene 1 and can be provided on an infecting bacteriophage λ vector or produced from a gene copy inserted into the E. coli chromosome ( Tabor and Richardson 1985 ; Studier and Moffatt 1986 ) . If expression of the cloned gene product is ...
... gene 1 and can be provided on an infecting bacteriophage λ vector or produced from a gene copy inserted into the E. coli chromosome ( Tabor and Richardson 1985 ; Studier and Moffatt 1986 ) . If expression of the cloned gene product is ...
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Common terms and phrases
acetate acrylamide activity Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA Biol blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted equal volume Escherichia coli ethanol ethidium bromide expression extract final concentration gene H H OH Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel Sephadex sequences single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml