Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
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Page xvii
... Genomic DNA Vectors Used to Construct Eukaryotic Genomic DNA Libraries 9.4 Bacteriophage λ and Cosmid Vectors 9.4 Yeast Artificial ... DNA Library 9.30 10 Analysis of Genomic DNA by Southern Hybridization 9.31 SEPARATION Contents Xvii.
... Genomic DNA Vectors Used to Construct Eukaryotic Genomic DNA Libraries 9.4 Bacteriophage λ and Cosmid Vectors 9.4 Yeast Artificial ... DNA Library 9.30 10 Analysis of Genomic DNA by Southern Hybridization 9.31 SEPARATION Contents Xvii.
Page xviii
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 10 Analysis of Genomic DNA by Southern Hybridization 9.31 SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. 10 Analysis of Genomic DNA by Southern Hybridization 9.31 SEPARATION OF RESTRICTION FRAGMENTS OF MAMMALIAN GENOMIC DNA BY AGAROSE GEL ELECTROPHORESIS 9.32 TRANSFER OF DNA FROM AGAROSE ...
Page 16-71
... genomic DNA results in uptake by the recipient cells of considerably more DNA than the DNA of the desired gene . Frequently , 0.01-0.1 % of the genome equivalent of DNA may be taken up by recipient cells , depending on the cell type ...
... genomic DNA results in uptake by the recipient cells of considerably more DNA than the DNA of the desired gene . Frequently , 0.01-0.1 % of the genome equivalent of DNA may be taken up by recipient cells , depending on the cell type ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml