Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-15
... Growth of wild - type or transfected mammalian cells in the presence of increasing concentrations of PALA leads to the amplification of the CAD gene and DNA sequences linked to it ( Kempe et al . 1976 ; Robert de Saint Vincent et al ...
... Growth of wild - type or transfected mammalian cells in the presence of increasing concentrations of PALA leads to the amplification of the CAD gene and DNA sequences linked to it ( Kempe et al . 1976 ; Robert de Saint Vincent et al ...
Page 16-35
... growth medium and the time of treatment are limited by the sensitivity of the cells to the toxic effect of the drug . The optimal concentration of chloroquine for the particular cell type that is used should therefore be determined in ...
... growth medium and the time of treatment are limited by the sensitivity of the cells to the toxic effect of the drug . The optimal concentration of chloroquine for the particular cell type that is used should therefore be determined in ...
Page A-9
... growth of phagemids . It makes high levels of lac repressor and is used for inducible expression of genes that are under the control of the lac promoter . This strain can be used for detection of recombinants expressing B ...
... growth of phagemids . It makes high levels of lac repressor and is used for inducible expression of genes that are under the control of the lac promoter . This strain can be used for detection of recombinants expressing B ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml