Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 56
Page xx
... Hybridization of Oligonucleotide Probes 11.45 CALCULATING MELTING TEMPERATURES FOR PERFECTLY MATCHED HYBRIDS BETWEEN OLIGONUCLEOTIDES AND THEIR TARGET SEQUENCES 11.46 ESTIMATING THE EFFECTS OF MISMATCHES 11.47 HYBRIDIZATION OF POOLS OF ...
... Hybridization of Oligonucleotide Probes 11.45 CALCULATING MELTING TEMPERATURES FOR PERFECTLY MATCHED HYBRIDS BETWEEN OLIGONUCLEOTIDES AND THEIR TARGET SEQUENCES 11.46 ESTIMATING THE EFFECTS OF MISMATCHES 11.47 HYBRIDIZATION OF POOLS OF ...
Page 1-18
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
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... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml