Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 58
Page xx
... Hybridization of Oligonucleotide Probes 11.45 CALCULATING MELTING TEMPERATURES FOR PERFECTLY MATCHED HYBRIDS BETWEEN OLIGONUCLEOTIDES AND THEIR TARGET SEQUENCES 11.46 ESTIMATING THE EFFECTS OF MISMATCHES 11.47 HYBRIDIZATION OF POOLS OF ...
... Hybridization of Oligonucleotide Probes 11.45 CALCULATING MELTING TEMPERATURES FOR PERFECTLY MATCHED HYBRIDS BETWEEN OLIGONUCLEOTIDES AND THEIR TARGET SEQUENCES 11.46 ESTIMATING THE EFFECTS OF MISMATCHES 11.47 HYBRIDIZATION OF POOLS OF ...
Page 1-18
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
... hybridization probes , 11.52-11.53 labeling , 11.15-11.16 3H , isotopic data , B.8 H HAT ( hypoxanthine , aminopterin , and thymidine ) medium , use as selec- tive agent , 16.9 , 16.14 HB101 , E. coli strain genotype and uses , A.10 ...
Page 1-29
... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
... hybridization and autoradiography , 7.52 staining RNA , 7.42 , 7.44-7.45 , 7.51 transfer of RNA to solid supports ... Hybridization activated , for western blotting , 18.64 capillary transfer of nucleic acids to supports , 7.49-7.50 ...
Other editions - View all
Common terms and phrases
acetate acrylamide activity Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA Biol blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted equal volume Escherichia coli ethanol ethidium bromide expression extract final concentration gene H H OH Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel Sephadex sequences single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml