Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 43
Page 17-36
... increasing the synthesis of foreign proteins . These include : Use of site - directed mutagenesis to increase translation initiation . The presence of secondary structure involving the ribosome - binding site can reduce the efficiency ...
... increasing the synthesis of foreign proteins . These include : Use of site - directed mutagenesis to increase translation initiation . The presence of secondary structure involving the ribosome - binding site can reduce the efficiency ...
Page 18-27
... increase the efficiency of incorporation of radiolabeled amino acids , it is necessary to incubate the cells in medium that is depleted of either methionine or both methionine and cysteine . Rapidly growing cells incorporate more ...
... increase the efficiency of incorporation of radiolabeled amino acids , it is necessary to incubate the cells in medium that is depleted of either methionine or both methionine and cysteine . Rapidly growing cells incorporate more ...
Page 18-44
... increasing quantities of antibody are used to precipitate a fixed amount of antigen . Typically , complete immunoprecipitation of ... increase . Dilution of the cell extract to 0.5 ml with NET - gel buffer lowers the level of nonspecific ...
... increasing quantities of antibody are used to precipitate a fixed amount of antigen . Typically , complete immunoprecipitation of ... increase . Dilution of the cell extract to 0.5 ml with NET - gel buffer lowers the level of nonspecific ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml