Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 54
Page xiv
... ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for ... ISOLATION OF RNAS 7.6 Isolation of Total RNA from Mammalian Cells 7.6 Rapid Isolation of Total RNA from Mammalian Cells ...
... ISOLATION OF INTACT DNA FROM YEAST 6.53 RESTRICTION ENZYME DIGESTION OF DNA IN AGAROSE BLOCKS 6.57 Markers for ... ISOLATION OF RNAS 7.6 Isolation of Total RNA from Mammalian Cells 7.6 Rapid Isolation of Total RNA from Mammalian Cells ...
Page 16-68
... isolation of eukaryotic genes . In contrast to bacterial cells , which are very useful in expressing immunologically ... isolated and assayed , or ( 3 ) the presentation on the cell surface of novel proteins that can be identified either ...
... isolation of eukaryotic genes . In contrast to bacterial cells , which are very useful in expressing immunologically ... isolated and assayed , or ( 3 ) the presentation on the cell surface of novel proteins that can be identified either ...
Page 16-70
... isolation of genes , they provided the experimental support for the concept that in many cases a given gene introduced into the appropriate cellular environment could be expressed and the gene product detected . The first genes to be ...
... isolation of genes , they provided the experimental support for the concept that in many cases a given gene introduced into the appropriate cellular environment could be expressed and the gene product detected . The first genes to be ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml