Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xxv
... Linker 15.86 Insertion of Hexameric Linkers 15.88 CREATING MANY MUTATIONS IN A DEFINED SEGMENT OF DNA 15.95 Use of Degenerate Pools of Mutagenic Oligonucleotides 15.96 Treatment of Double - stranded DNA with Chemical Mutagens 15.105 ...
... Linker 15.86 Insertion of Hexameric Linkers 15.88 CREATING MANY MUTATIONS IN A DEFINED SEGMENT OF DNA 15.95 Use of Degenerate Pools of Mutagenic Oligonucleotides 15.96 Treatment of Double - stranded DNA with Chemical Mutagens 15.105 ...
Page xxxii
... Linkers F.8 Ligation of Phosphorylated Linkers to Blunt - ended Target Fragments F.9 Appendix G : List of Suppliers References Molecular Cloning Expression of Cloned Genes in Cultured Mammalian Cells Xxxii Contents.
... Linkers F.8 Ligation of Phosphorylated Linkers to Blunt - ended Target Fragments F.9 Appendix G : List of Suppliers References Molecular Cloning Expression of Cloned Genes in Cultured Mammalian Cells Xxxii Contents.
Page F-1
... linkers to blunt - ended DNA . Synthetic linkers are an equimolar mixture of self - complementary , chemically synthesized oligomers that can form blunt - ended duplexes containing one or more recognition sites for restriction enzymes ...
... linkers to blunt - ended DNA . Synthetic linkers are an equimolar mixture of self - complementary , chemically synthesized oligomers that can form blunt - ended duplexes containing one or more recognition sites for restriction enzymes ...
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Common terms and phrases
acetate acrylamide activity Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA Biol blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted equal volume Escherichia coli ethanol ethidium bromide expression extract final concentration gene H H OH Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel Sephadex sequences single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml