Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 83
Page 16-60
... minutes at 4 ° C in a microfuge . Transfer the supernatant to a fresh microfuge tube . Reserve 50 μl for the CAT assay , and store the remainder of the extract at -20 ° C . 4. Incubate the 50 - μl aliquot of the extract for 10 minutes ...
... minutes at 4 ° C in a microfuge . Transfer the supernatant to a fresh microfuge tube . Reserve 50 μl for the CAT assay , and store the remainder of the extract at -20 ° C . 4. Incubate the 50 - μl aliquot of the extract for 10 minutes ...
Page 17-19
... minutes at 37 ° C . c . Heat the reaction for 5 minutes at 68 ° C to inactivate the bacteriophage T4 polynucleotide kinase . Place on dry ice and store at -20 ° C . 3. Hybridize the primer to template bacteriophage M13 single - stranded ...
... minutes at 37 ° C . c . Heat the reaction for 5 minutes at 68 ° C to inactivate the bacteriophage T4 polynucleotide kinase . Place on dry ice and store at -20 ° C . 3. Hybridize the primer to template bacteriophage M13 single - stranded ...
Page 18-37
... minutes . 7. Collect the protoplasts by centrifugation at 500g for 10 minutes at 4 ° C . Discard the supernatant . 8. Resuspend the pellet ( which consists of protoplasts and intact cells ) in a volume of lysis buffer equivalent to 0.01 ...
... minutes . 7. Collect the protoplasts by centrifugation at 500g for 10 minutes at 4 ° C . Discard the supernatant . 8. Resuspend the pellet ( which consists of protoplasts and intact cells ) in a volume of lysis buffer equivalent to 0.01 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml