Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-34
... mixture for 20-30 minutes at room temperature , during which time a fine precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used alternative procedure ...
... mixture for 20-30 minutes at room temperature , during which time a fine precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used alternative procedure ...
Page 16-63
... mixture are : 2.3 mM chloramphenicol , 0.11 M Tris Cl , and 129 μM ( 1 μCi / ml ) 1C - labeled acetyl coenzyme A. 14 · For each 30 μl of cell extract to be tested , 70 μl of CAT reaction mixture 2 is required . 2. Add 30 μl of cell ...
... mixture are : 2.3 mM chloramphenicol , 0.11 M Tris Cl , and 129 μM ( 1 μCi / ml ) 1C - labeled acetyl coenzyme A. 14 · For each 30 μl of cell extract to be tested , 70 μl of CAT reaction mixture 2 is required . 2. Add 30 μl of cell ...
Page 16-64
... mixture for 15 minutes at 65 ° C to inactivate deacetylases in the extract . Transfer the vial to a water bath set at 37 ° C . 2. Add 200 μl of freshly prepared CAT reaction mixture 3 : 1 M Tris Cl ( pH 7.8 ) 5 mm chloramphenicol 3H ...
... mixture for 15 minutes at 65 ° C to inactivate deacetylases in the extract . Transfer the vial to a water bath set at 37 ° C . 2. Add 200 μl of freshly prepared CAT reaction mixture 3 : 1 M Tris Cl ( pH 7.8 ) 5 mm chloramphenicol 3H ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml