Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 29
Page xxiv
... Mutants 15.14 GENERATION OF BIDIRECTIONAL SETS OF DELETION MUTANTS BY DIGESTION WITH NUCLEASE BAL 31 15.20 CLEAVAGE OF DOUBLE - STRANDED CLOSED CIRCULAR DNA WITH PANCREATIC DNAase I IN THE PRESENCE OF Mn ** 15.27 Linker - scanning ...
... Mutants 15.14 GENERATION OF BIDIRECTIONAL SETS OF DELETION MUTANTS BY DIGESTION WITH NUCLEASE BAL 31 15.20 CLEAVAGE OF DOUBLE - STRANDED CLOSED CIRCULAR DNA WITH PANCREATIC DNAase I IN THE PRESENCE OF Mn ** 15.27 Linker - scanning ...
Page 16-56
... mutant versions of putative cis - acting controlling elements is measured in transfect- ed mammalian cells . The ... mutants must be purified and analyzed by hybridization and gel electrophoresis . In most cases , therefore , the ...
... mutant versions of putative cis - acting controlling elements is measured in transfect- ed mammalian cells . The ... mutants must be purified and analyzed by hybridization and gel electrophoresis . In most cases , therefore , the ...
Page 1-28
... mutants using BAL 31 , 15.14-15.15 , 15.20- 15.21 , 15.22-15.26 using DNAase I , 15.14 , 15.16 , 15.27-15.31 using exonuclease III , 5.84 , 13.34 , 13.37 , 13.39-13.41 , 15.14 , 15.17-15.19 by misincorporation of nucleotides by DNA ...
... mutants using BAL 31 , 15.14-15.15 , 15.20- 15.21 , 15.22-15.26 using DNAase I , 15.14 , 15.16 , 15.27-15.31 using exonuclease III , 5.84 , 13.34 , 13.37 , 13.39-13.41 , 15.14 , 15.17-15.19 by misincorporation of nucleotides by DNA ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml