Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 28
Page 1-12
... mutations by misincor- poration of nucleotides , 15.108 generation of blunt - ended cDNA , 8.23 nick translation of DNA , 10.6-10.12 second - strand cDNA synthesis , 8.14- 8.20 , 8.64 Klenow fragment ( large fragment ) , reac- tions and ...
... mutations by misincor- poration of nucleotides , 15.108 generation of blunt - ended cDNA , 8.23 nick translation of DNA , 10.6-10.12 second - strand cDNA synthesis , 8.14- 8.20 , 8.64 Klenow fragment ( large fragment ) , reac- tions and ...
Page 1-21
... mutation , 4.12 A ( lac - proAB ) , deletion of lac operon , 4.12 A ( lac - proAB ) , deletion of lac operon , ge- netic ... mutations , 9.8 ligation to fragments of foreign DNA , 2.94 purification , 2.85-2.89 assembly pathway , 2.7 att ...
... mutation , 4.12 A ( lac - proAB ) , deletion of lac operon , 4.12 A ( lac - proAB ) , deletion of lac operon , ge- netic ... mutations , 9.8 ligation to fragments of foreign DNA , 2.94 purification , 2.85-2.89 assembly pathway , 2.7 att ...
Page 1-38
... mutations , 2.55 , 2.57 , 4.13 hsdS , genetic marker in E. coli host strain , 2.55 , 2.57 methylation by E. coli strains , 5.15– 5.17 methylation sensitivity differences between isoschizomer pairs , 5.17 , 5.25 of restriction enzymes ...
... mutations , 2.55 , 2.57 , 4.13 hsdS , genetic marker in E. coli host strain , 2.55 , 2.57 methylation by E. coli strains , 5.15– 5.17 methylation sensitivity differences between isoschizomer pairs , 5.17 , 5.25 of restriction enzymes ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml