Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 11
Page 18-17
... filter as described on pages 18.64-18.66 . 2. After transfer , incubate the filter in blocking buffer for 1 hour at room temperature . Use 0.25 ml of blocking buffer for each square centimeter of ... NITROCELLULOSE FILTERS 18 17.
... filter as described on pages 18.64-18.66 . 2. After transfer , incubate the filter in blocking buffer for 1 hour at room temperature . Use 0.25 ml of blocking buffer for each square centimeter of ... NITROCELLULOSE FILTERS 18 17.
Page 18-66
... nitrocellulose filter . Orient the gel so that the mark on the filter corresponds to the bottom left - hand corner of the gel . Squeeze out any trapped air bubbles with a gloved hand . Important : To avoid the possibility of a short ...
... nitrocellulose filter . Orient the gel so that the mark on the filter corresponds to the bottom left - hand corner of the gel . Squeeze out any trapped air bubbles with a gloved hand . Important : To avoid the possibility of a short ...
Page 18-68
... nitrocellulose filter has been dried , float it on the surface of a tray of deionized water and allow it to wet from beneath by capillary action . Then , submerge the filter in the water for at least 5 minutes to displace trapped air ...
... nitrocellulose filter has been dried , float it on the surface of a tray of deionized water and allow it to wet from beneath by capillary action . Then , submerge the filter in the water for at least 5 minutes to displace trapped air ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml