Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 225
... (FILTER ASSAY ) This assay can be used to assay the grid of positives restreaked to selective media in the previous step. If there are a large number of positive colonies that make restreaking prohibitive, nitrocellulose filters can be ...
... (FILTER ASSAY ) This assay can be used to assay the grid of positives restreaked to selective media in the previous step. If there are a large number of positive colonies that make restreaking prohibitive, nitrocellulose filters can be ...
Page 318
... nitrocellulose sheet (cut to the same size as the gel) in transfer buffer, and place it on the gel, i.e., on the anodal side (see Note 4). Avoid trapping air bubbles throughout the process. 4. Place the remaining filter paper over the ...
... nitrocellulose sheet (cut to the same size as the gel) in transfer buffer, and place it on the gel, i.e., on the anodal side (see Note 4). Avoid trapping air bubbles throughout the process. 4. Place the remaining filter paper over the ...
Page 484
... filter binding assay (Tanaka and Schwer, 2005; Wong and Lohman, 1993), which involves rapidly passing the preincubated protein-RNA mixture through two filters: nitrocellulose on top and nylon on the bottom. Protein-bound RNA is retained ...
... filter binding assay (Tanaka and Schwer, 2005; Wong and Lohman, 1993), which involves rapidly passing the preincubated protein-RNA mixture through two filters: nitrocellulose on top and nylon on the bottom. Protein-bound RNA is retained ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml