Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 58
Page 16-28
... obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign gene is attached to a segment of DNA that carries a drug resistance marker and transfected into the cells , or the ...
... obtained from lines of cells carrying amplified chromosomal copies of the gene of interest . In this method , the foreign gene is attached to a segment of DNA that carries a drug resistance marker and transfected into the cells , or the ...
Page 16-55
... obtained when linear DNA is used ( Neumann et al . 1982 ; Potter et al . 1984 ; Toneguzzo et al . 1986 ) . Effective transfection has been obtained with concentrations of DNA rang- ing from 1 μg / ml to 40 μg / ml . • Ionic composition ...
... obtained when linear DNA is used ( Neumann et al . 1982 ; Potter et al . 1984 ; Toneguzzo et al . 1986 ) . Effective transfection has been obtained with concentrations of DNA rang- ing from 1 μg / ml to 40 μg / ml . • Ionic composition ...
Page 18-21
... obtained with RIAs are the sensitivity , accuracy , and specificity of the assay . The sensitivity of the assay is determined by two factors : • The amount of the first antibody that is bound to the solid support ( usually a 96 - well ...
... obtained with RIAs are the sensitivity , accuracy , and specificity of the assay . The sensitivity of the assay is determined by two factors : • The amount of the first antibody that is bound to the solid support ( usually a 96 - well ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml