Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xix
... Oligonucleotide Probes Types and Uses of Oligonucleotide Probes 11.3 SINGLE OLIGONUCLEOTIDES OF DEFINED SEQUENCE 11.4 POOLS OF SHORT OLIGONUCLEOTIDES WHOSE SEQUENCES ARE HIGHLY DEGENERATE 11.5 The Effects of Length and Degeneracy of the ...
... Oligonucleotide Probes Types and Uses of Oligonucleotide Probes 11.3 SINGLE OLIGONUCLEOTIDES OF DEFINED SEQUENCE 11.4 POOLS OF SHORT OLIGONUCLEOTIDES WHOSE SEQUENCES ARE HIGHLY DEGENERATE 11.5 The Effects of Length and Degeneracy of the ...
Page xx
... Oligonucleotide on the Specificity of Hybridization 11.7 Designing Degenerate Pools of Short Oligonucleotides 11.9 POOLS OF LONGER OLIGONUCLEOTIDES OF LESSER DEGENERACY Guessmers 11.11 11.11 11.11 DESIGNING A GUESSMER LABELING OF ...
... Oligonucleotide on the Specificity of Hybridization 11.7 Designing Degenerate Pools of Short Oligonucleotides 11.9 POOLS OF LONGER OLIGONUCLEOTIDES OF LESSER DEGENERACY Guessmers 11.11 11.11 11.11 DESIGNING A GUESSMER LABELING OF ...
Page xxv
... Oligonucleotide - mediated Mutagenesis 15.61 OLIGONUCLEOTIDE - MEDIATED MUTAGENESIS BY THE DOUBLE PRIMER METHOD 15.63 SCREENING BACTERIOPHAGE M13 PLAQUES AND COLONIES BY HYBRIDIZATION TO RADIOLABELED OLIGONUCLEOTIDES 15.66 Radiolabeling ...
... Oligonucleotide - mediated Mutagenesis 15.61 OLIGONUCLEOTIDE - MEDIATED MUTAGENESIS BY THE DOUBLE PRIMER METHOD 15.63 SCREENING BACTERIOPHAGE M13 PLAQUES AND COLONIES BY HYBRIDIZATION TO RADIOLABELED OLIGONUCLEOTIDES 15.66 Radiolabeling ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml