Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 18
Page ix
... Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro 2.98 PROTOCOL I : PREPARATION OF PACKAGING EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING ...
... Packaging Extracts and Packaging of Bacteriophage λ DNA In Vitro 2.98 PROTOCOL I : PREPARATION OF PACKAGING EXTRACTS FROM TWO LYSOGENS 2.100 PROTOCOL I : PACKAGING IN VITRO USING TWO EXTRACTS 2.104 PROTOCOL II : PREPARATION OF PACKAGING ...
Page A-9
... packaging extracts ( Hohn and Murray 1977 ; Hohn 1979 ) . A bacteriophage λ lysogen used to prepare packaging extracts ( Hohn and Murray 1977 ; Hohn 1979 ) . A strain employed for high - level expression of genes cloned into expression ...
... packaging extracts ( Hohn and Murray 1977 ; Hohn 1979 ) . A bacteriophage λ lysogen used to prepare packaging extracts ( Hohn and Murray 1977 ; Hohn 1979 ) . A strain employed for high - level expression of genes cloned into expression ...
Page 1-17
... packaging , 3.52 packaging protocol , 3.52-3.53 partial digestion with Mbol and Sau3AI , 3.32 , 9.24-9.28 size fractionation versus dephosphory- lation , 3.35 , 3.38 expression libraries , 2.13 , 2.42-2.54 , 12.6-12.7 , 12.10 , 16.70 ...
... packaging , 3.52 packaging protocol , 3.52-3.53 partial digestion with Mbol and Sau3AI , 3.32 , 9.24-9.28 size fractionation versus dephosphory- lation , 3.35 , 3.38 expression libraries , 2.13 , 2.42-2.54 , 12.6-12.7 , 12.10 , 16.70 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml