Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-18
... particles . In rodent cells , infection is blocked at the level of DNA replication . The viral genome is a covalently closed circular double - stranded DNA molecule of 5243 bp . The genome is functionally divided into early and late ...
... particles . In rodent cells , infection is blocked at the level of DNA replication . The viral genome is a covalently closed circular double - stranded DNA molecule of 5243 bp . The genome is functionally divided into early and late ...
Page E-21
... particles . However , the energies of these particles are very different : 35S emits a particle with a maximum energy of 0.167 MeV that can penetrate film emulsion only to a depth of 0.25 mm ( see Figure E.3 ) . Although this is ...
... particles . However , the energies of these particles are very different : 35S emits a particle with a maximum energy of 0.167 MeV that can penetrate film emulsion only to a depth of 0.25 mm ( see Figure E.3 ) . Although this is ...
Page E-22
... Particle energy ( MeV ) 1.5 32p 1.710 double - sided X - ray film Energy of radiation emitted by commonly used isotopes . The graphs in the upper part of the figure show the spectra of energies carried by particles emitted by decaying ...
... Particle energy ( MeV ) 1.5 32p 1.710 double - sided X - ray film Energy of radiation emitted by commonly used isotopes . The graphs in the upper part of the figure show the spectra of energies carried by particles emitted by decaying ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml