Molecular Cloning: A Laboratory Manual, Book 3 |
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Page v
... Plasmid Vectors Essential Features of Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical ...
... Plasmid Vectors Essential Features of Plasmids 1.3 Replication and Incompatibility 1.3 Mobilization 1.5 Selectable Markers 1.5 Plasmid Vectors 1.7 DEVELOPMENT OF PLASMID CLONING VECTORS 1.7 Plasmid Vectors That Permit Histochemical ...
Page vi
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
... Plasmid DNA 1.31 Rapid Disruption of Bacterial Colonies to Test the Size of Plasmids 1.32 LARGE - SCALE PREPARATIONS OF PLASMID DNA 1.33 Amplification of Plasmids in Rich Medium 1.33 Harvesting and Lysis of Bacteria 1.34 HARVESTING 1.34 ...
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... Plasmid amplification by chloramphenicol , 1.3 , 1.21 , 1.23 , 1.33 cloning of DNA in plasmid vectors , 1.53 availability of appropriate restriction sites , 1.59 dephosphorylation of linear plasmid DNA , 1.60-1.62 determining extent of ...
... Plasmid amplification by chloramphenicol , 1.3 , 1.21 , 1.23 , 1.33 cloning of DNA in plasmid vectors , 1.53 availability of appropriate restriction sites , 1.59 dephosphorylation of linear plasmid DNA , 1.60-1.62 determining extent of ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml