Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 31
Page 16-61
... plate in the chromatography chamber , close the chamber , and allow the solvent front to move approximately 75 % of the distance to the top of the plate . 14. Remove the TLC plate from the tank and allow it to dry at room temperature ...
... plate in the chromatography chamber , close the chamber , and allow the solvent front to move approximately 75 % of the distance to the top of the plate . 14. Remove the TLC plate from the tank and allow it to dry at room temperature ...
Page 18-22
... plate contains . 2. To each well of a PVC plate , add 50 μl of IgG solution ( 20 μg / ml in 0.2 M NaHCO3 ) . Cover the plate , and incubate it for 2 hours at room tempera- ture in a humidified atmosphere . A typical humidified tissue ...
... plate contains . 2. To each well of a PVC plate , add 50 μl of IgG solution ( 20 μg / ml in 0.2 M NaHCO3 ) . Cover the plate , and incubate it for 2 hours at room tempera- ture in a humidified atmosphere . A typical humidified tissue ...
Page 18-23
... plate that are not occupied by immunoglobulin molecules . The antibody - coated plates may be stored for several ... plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C . 8. Wash the ...
... plate that are not occupied by immunoglobulin molecules . The antibody - coated plates may be stored for several ... plate and incubate it for 2 hours at room temperature in a humidified atmo- sphere or for 8 hours at 4 ° C . 8. Wash the ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml