Molecular Cloning: A Laboratory Manual, Book 3 |
From inside the book
Results 1-3 of 13
Page 16-16
... positions -3 and +4 . As long as there is a purine in position -3 , deviations from the rest of the consensus sequence only marginally impair initiation . In the absence of a purine in position -3 , however , G ** is essential for ...
... positions -3 and +4 . As long as there is a purine in position -3 , deviations from the rest of the consensus sequence only marginally impair initiation . In the absence of a purine in position -3 , however , G ** is essential for ...
Page 18-51
... position at room temperature . The overlay prevents oxygen from diffusing into the gel and inhibiting polymeri- zation . 4. After polymerization is complete ( 30 minutes ) , pour off the overlay and wash the top of the gel several times ...
... position at room temperature . The overlay prevents oxygen from diffusing into the gel and inhibiting polymeri- zation . 4. After polymerization is complete ( 30 minutes ) , pour off the overlay and wash the top of the gel several times ...
Page D-1
... position of codon U ט כ с U A G บ บ บ Phe UUC Phe UCU Ser UAU Tyr UGU Cys UCC Ser UAC Tyr UGC Cys U UUA Leu UUG Leu ... position of codon ( 5 ' terminus ) A AG ט כ AUU lle ACU Thr AAU Asn AGU Ser AUC lle ACC Thr AAC Asn AGC Ser AUA lle ...
... position of codon U ט כ с U A G บ บ บ Phe UUC Phe UCU Ser UAU Tyr UGU Cys UCC Ser UAC Tyr UGC Cys U UUA Leu UUG Leu ... position of codon ( 5 ' terminus ) A AG ט כ AUU lle ACU Thr AAU Asn AGU Ser AUC lle ACC Thr AAC Asn AGC Ser AUA lle ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml