Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 32
Page 16-34
... precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used alternative procedure is to add a dilute mixture of CaCl , and DNA to the 2 × HBS solution ...
... precipitate should form . At the end of the incubation , pipette the mixture up and down once to resuspend the precipitate . A commonly used alternative procedure is to add a dilute mixture of CaCl , and DNA to the 2 × HBS solution ...
Page 16-39
... precipitates gradually onto the cells during the incubation in an atmosphere containing low concentrations of CO2 . The nature of the precipitate is affected by the amount of DNA used . A transition from a coarse precipitate to a fine ...
... precipitates gradually onto the cells during the incubation in an atmosphere containing low concentrations of CO2 . The nature of the precipitate is affected by the amount of DNA used . A transition from a coarse precipitate to a fine ...
Page E-10
... precipitate of nucleic acid , which is allowed to form in the presence of moderate concentrations of monovalent cations , is recovered by centrifugation and redissolved in an appropriate buffer at the desired concen- tration . The ...
... precipitate of nucleic acid , which is allowed to form in the presence of moderate concentrations of monovalent cations , is recovered by centrifugation and redissolved in an appropriate buffer at the desired concen- tration . The ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml