Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xxxii
... Precipitation with Ethanol or Isopropanol E.10 PRECIPITATION OF DNA IN MICROFUGE TUBES E.12 PRECIPITATION OF RNA WITH ETHANOL E.15 PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE E.15 CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH ...
... Precipitation with Ethanol or Isopropanol E.10 PRECIPITATION OF DNA IN MICROFUGE TUBES E.12 PRECIPITATION OF RNA WITH ETHANOL E.15 PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE E.15 CONCENTRATING NUCLEIC ACIDS BY EXTRACTION WITH ...
Page E-10
A Laboratory Manual Joseph Sambrook, Tom Maniatis. CONCENTRATING NUCLEIC ACIDS Precipitation with Ethanol or Isopropanol The most widely used method for concentrating nucleic acids is precipitation with ethanol . The precipitate of ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. CONCENTRATING NUCLEIC ACIDS Precipitation with Ethanol or Isopropanol The most widely used method for concentrating nucleic acids is precipitation with ethanol . The precipitate of ...
Page E-15
... precipitation of RNA should be free of RNAase ( see Chapter 7 , page 7.4 ) . PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE Whereas small RNAs ( tRNAs and 5S RNAs ) are soluble in solutions of high ionic strength , large RNAs ( e.g. ...
... precipitation of RNA should be free of RNAase ( see Chapter 7 , page 7.4 ) . PRECIPITATION OF LARGE RNAS WITH LITHIUM CHLORIDE Whereas small RNAs ( tRNAs and 5S RNAs ) are soluble in solutions of high ionic strength , large RNAs ( e.g. ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml