Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 16-58
... prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli ẞ - galactosidase , which is expressed in transfected mammalian cells from a promoter with a broad host range ( the SV40 early promoter or ...
... prepared for measurement of CAT activity . An enzyme frequently used for this purpose is E. coli ẞ - galactosidase , which is expressed in transfected mammalian cells from a promoter with a broad host range ( the SV40 early promoter or ...
Page 17-25
... prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described on pages ...
... prepared by one of the methods described above ( see pages 17.18-17.24 ) . In most cases , a blunt - ended fragment will be inserted and this step will not be required . In a few cases , the gene fragment prepared as described on pages ...
Page 18-49
... prepared in deionized , warm water ( to assist the dissolution of the bisacrylamide ) . Acrylamide and bisacrylamide are slowly converted during storage to acrylic acid and bisacrylic acid . This deamination reaction is catalyzed by ...
... prepared in deionized , warm water ( to assist the dissolution of the bisacrylamide ) . Acrylamide and bisacrylamide are slowly converted during storage to acrylic acid and bisacrylic acid . This deamination reaction is catalyzed by ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml