Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 39
Page 16-15
... presence of increasing concentrations of PALA leads to the amplification of the CAD gene and DNA sequences linked to it ( Kempe et al . 1976 ; Robert de Saint Vincent et al . 1981 ; Wahl et al . 1984 ) . The E. coli gene encoding ...
... presence of increasing concentrations of PALA leads to the amplification of the CAD gene and DNA sequences linked to it ( Kempe et al . 1976 ; Robert de Saint Vincent et al . 1981 ; Wahl et al . 1984 ) . The E. coli gene encoding ...
Page 18-27
... presence of 35 S - labeled sulfate ( e.g. , Tran35 S - label ; ICN Radiochemicals ) that can be used as a substitute for [ 35S ] Met in metabolic labeling . About 70 % of the label in the hydrolysate is in the form of 35S ] Met , with ...
... presence of 35 S - labeled sulfate ( e.g. , Tran35 S - label ; ICN Radiochemicals ) that can be used as a substitute for [ 35S ] Met in metabolic labeling . About 70 % of the label in the hydrolysate is in the form of 35S ] Met , with ...
Page 18-78
... presence of EGTA or by the presence of inactive micrococcal nuclease . ii . Hemin is included in the reticulocyte lysate because it is a powerful suppressor of an inhibitor of the initiation factor eIF - 2 . In the absence of hemin ...
... presence of EGTA or by the presence of inactive micrococcal nuclease . ii . Hemin is included in the reticulocyte lysate because it is a powerful suppressor of an inhibitor of the initiation factor eIF - 2 . In the absence of hemin ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml