Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 47
Page xviii
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
... PROBES TO IMMOBILIZED NUCLEIC ACIDS 9.47 Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Nitrocellulose Filters or Nylon Membranes 9.52 Hybridization of Radiolabeled Oligonucleotides to Genomic DNA 9.56 Removal of ...
Page xix
... Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES 10.38 Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING 10.38 ABSORBED PROBES 10.40 SUBTRACTED LIBRARIES 10.40 SUBTRACTED PROBES ...
... Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF CDNA PROBES 10.38 Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING 10.38 ABSORBED PROBES 10.40 SUBTRACTED LIBRARIES 10.40 SUBTRACTED PROBES ...
Page 1-29
... probes , 7.62-7.65 using single - stranded DNA probes , 7.66–7.70 Nucleoside analogs used as chain- terminators in DNA sequencing , structures , C.13 . See also Dideoxy- mediated chain - termination ( Sanger ) method for DNA se- quence ...
... probes , 7.62-7.65 using single - stranded DNA probes , 7.66–7.70 Nucleoside analogs used as chain- terminators in DNA sequencing , structures , C.13 . See also Dideoxy- mediated chain - termination ( Sanger ) method for DNA se- quence ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml