Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-2
... protein from a cloned gene introduced into Escherichia coli have proven invaluable in the purification , localization , and functional analysis of proteins . For example , fusion proteins consisting of amino - terminal peptides encoded ...
... protein from a cloned gene introduced into Escherichia coli have proven invaluable in the purification , localization , and functional analysis of proteins . For example , fusion proteins consisting of amino - terminal peptides encoded ...
Page 17-2
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
... proteins . These antibodies have been used ( 1 ) to purify proteins by immunoaffinity chromatography ; ( 2 ) in diagnostic assays to quantitate the levels of protein ; and ( 3 ) to localize the proteins in organisms , tissues , and ...
Page 17-29
... protein not have an amino - terminal methionine , which is coded for by the ATG in these vectors . The amino ... Protein That Can Be Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein ...
... protein not have an amino - terminal methionine , which is coded for by the ATG in these vectors . The amino ... Protein That Can Be Cleaved by a Protease or Cyanogen Bromide The cloned gene can be expressed as part of a fusion protein ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml