Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 17-30
... purify the fusion protein ( see Nagai and Thøgersen 1984 ) . Store at -20 ° C until needed . a d . Activate bovine blood coagulation factor X to factor X , with Russell's viper venom ( Fujikawa et al . 1972 ) . a e . Dissolve the purified ...
... purify the fusion protein ( see Nagai and Thøgersen 1984 ) . Store at -20 ° C until needed . a d . Activate bovine blood coagulation factor X to factor X , with Russell's viper venom ( Fujikawa et al . 1972 ) . a e . Dissolve the purified ...
Page 18-11
... purify the IgG fraction or , in some cases , to purify the antibody of interest by binding it to its cognate antigen ... purified by another method ( e.g. , ammonium sulfate precipitation followed by chromatography on DEAE - cellulose ) ...
... purify the IgG fraction or , in some cases , to purify the antibody of interest by binding it to its cognate antigen ... purified by another method ( e.g. , ammonium sulfate precipitation followed by chromatography on DEAE - cellulose ) ...
Page 18-16
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Purification of Immunospecific Antibodies Antibodies may be purified by adsorption to , and elution from , their cognate antigen . In some cases ( e.g. , when the antigen is a protein ) ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. Purification of Immunospecific Antibodies Antibodies may be purified by adsorption to , and elution from , their cognate antigen . In some cases ( e.g. , when the antigen is a protein ) ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml