Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 64
Page 17-20
... restriction site 3 PAS1 23 restriction site 3 promoter SD sequence 2 3 restriction site 3 restriction site. 3 lle restriction site 3 restriction site 1 1 2 Met Ala ATG | GCC | ATC 2 3 5 GCCATC 3. CGG TAG Treat with BAL 31 untranslated ...
... restriction site 3 PAS1 23 restriction site 3 promoter SD sequence 2 3 restriction site 3 restriction site. 3 lle restriction site 3 restriction site 1 1 2 Met Ala ATG | GCC | ATC 2 3 5 GCCATC 3. CGG TAG Treat with BAL 31 untranslated ...
Page 17-22
A Laboratory Manual Joseph Sambrook, Tom Maniatis. ENGINEERING A RESTRICTION SITE Place a restriction site immediately preceding the second codon ( Panayotatos and Truong 1981 ; Shatzman et al . 1983 ) . This can be done as follows ( see ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. ENGINEERING A RESTRICTION SITE Place a restriction site immediately preceding the second codon ( Panayotatos and Truong 1981 ; Shatzman et al . 1983 ) . This can be done as follows ( see ...
Page 1-38
... restriction mutations , 2.55 , 2.57 , 4.13 hsdS , genetic marker in E. coli host strain , 2.55 , 2.57 methylation by E. coli strains , 5.15– 5.17 methylation sensitivity differences between isoschizomer pairs , 5.17 , 5.25 of restriction ...
... restriction mutations , 2.55 , 2.57 , 4.13 hsdS , genetic marker in E. coli host strain , 2.55 , 2.57 methylation by E. coli strains , 5.15– 5.17 methylation sensitivity differences between isoschizomer pairs , 5.17 , 5.25 of restriction ...
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acetate acrylamide Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract final concentration fragment gene H H H H OH hybridization Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl NH3 H nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml