Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 62
Page 17-20
... restriction site 3 restriction site 1 Treat with BAL 31 5 ' sequences plasmid DNA restriction site 1 restriction site 3 restriction site 3 2 3 PAS1 recreated restriction site 1 restriction site 3 Blunt - end ligation restriction site 3 ...
... restriction site 3 restriction site 1 Treat with BAL 31 5 ' sequences plasmid DNA restriction site 1 restriction site 3 restriction site 3 2 3 PAS1 recreated restriction site 1 restriction site 3 Blunt - end ligation restriction site 3 ...
Page 17-22
A Laboratory Manual Joseph Sambrook, Tom Maniatis. ENGINEERING A RESTRICTION SITE Place a restriction site immediately preceding the second codon ( Panayotatos and Truong 1981 ; Shatzman et al . 1983 ) . This can be done as follows ( see ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. ENGINEERING A RESTRICTION SITE Place a restriction site immediately preceding the second codon ( Panayotatos and Truong 1981 ; Shatzman et al . 1983 ) . This can be done as follows ( see ...
Page 1-38
... restriction mutations , 2.55 , 2.57 , 4.13 hsdS , genetic marker in E. coli host strain , 2.55 , 2.57 methylation by E. coli strains , 5.15– 5.17 methylation sensitivity differences between isoschizomer pairs , 5.17 , 5.25 of restriction ...
... restriction mutations , 2.55 , 2.57 , 4.13 hsdS , genetic marker in E. coli host strain , 2.55 , 2.57 methylation by E. coli strains , 5.15– 5.17 methylation sensitivity differences between isoschizomer pairs , 5.17 , 5.25 of restriction ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml