Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 45
Page xxi
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
... SCREENING EXPRESSION LIBRARIES CONSTRUCTED IN BACTERIOPHAGE A VECTORS 12.16 SCREENING BACTERIAL COLONIES 12.21 Preparation of Colonies for Screening 12.21 METHOD 1 12.21 METHOD 2 12.23 Processing Filters for Immunological Screening of ...
Page xxv
... Screening Bacteriophage M13 Plaques by Hybridization to Radiolabeled Oligonucleotides 15.68 Screening Bacterial Colonies by Hybridization to Radiolabeled Oligonucleotides 15.72 OLIGONUCLEOTIDE - DIRECTED MUTAGENESIS BY SELECTION AGAINST ...
... Screening Bacteriophage M13 Plaques by Hybridization to Radiolabeled Oligonucleotides 15.68 Screening Bacterial Colonies by Hybridization to Radiolabeled Oligonucleotides 15.72 OLIGONUCLEOTIDE - DIRECTED MUTAGENESIS BY SELECTION AGAINST ...
Page 1-6
... screening by , 8.32-8.33 hybridization screening of libraries , 8.46-8.49 hybrid selection of clones , 8.50-8.51 identification and validation of clones , 8.51-8.52 methylase treatment of cDNA , 8.24 , 8.66-8.67 mRNA abundance and ...
... screening by , 8.32-8.33 hybridization screening of libraries , 8.46-8.49 hybrid selection of clones , 8.50-8.51 identification and validation of clones , 8.51-8.52 methylase treatment of cDNA , 8.24 , 8.66-8.67 mRNA abundance and ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml