Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 51
Page 16-10
... selection of stably transfected cells must be determined empirically . • Aminoglycoside phosphotransferase . The mostly widely used dominant selection system utilizes the bacterial gene encoding aminoglycoside 3 ' phosphotransferase ...
... selection of stably transfected cells must be determined empirically . • Aminoglycoside phosphotransferase . The mostly widely used dominant selection system utilizes the bacterial gene encoding aminoglycoside 3 ' phosphotransferase ...
Page 16-71
... selection or screening that is essential for the initial identification of cells that have taken up and express the desired gene . Several strategies have been used to actually identify and isolate the desired gene . Transformation with ...
... selection or screening that is essential for the initial identification of cells that have taken up and express the desired gene . Several strategies have been used to actually identify and isolate the desired gene . Transformation with ...
Page 1-2
... selection sys- tems , 16.10-16.14 . See also Anti- biotics ; Selection Aminopterin , selection , 3.18 , 16.9 . See also HAT medium ; Selection Ammonium acetate , stock solution , B.10 Ammonium hydroxide common commercial strengths , B.2 ...
... selection sys- tems , 16.10-16.14 . See also Anti- biotics ; Selection Aminopterin , selection , 3.18 , 16.9 . See also HAT medium ; Selection Ammonium acetate , stock solution , B.10 Ammonium hydroxide common commercial strengths , B.2 ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml