Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 32
Page 16-35
... selective medium for the isolation of stable transformants . b . In many instances , uptake of DNA is increased by concurrent treat- ment of the cells with chloroquine , which may act by inhibiting the degradation of the DNA by ...
... selective medium for the isolation of stable transformants . b . In many instances , uptake of DNA is increased by concurrent treat- ment of the cells with chloroquine , which may act by inhibiting the degradation of the DNA by ...
Page 16-36
... selective medium to allow expression of the transferred gene ( s ) to occur , the cells are trypsinized and replated in the appropriate selective medium . This medium should be changed every 2-4 days for 2-3 weeks to remove the debris ...
... selective medium to allow expression of the transferred gene ( s ) to occur , the cells are trypsinized and replated in the appropriate selective medium . This medium should be changed every 2-4 days for 2-3 weeks to remove the debris ...
Page 1-2
... selective agent , 1.5 for maintaining expression plasmids , 17.16 Amplification , 1.3 of bacteriophage › plaques on nitrocellu- lose filters , 2.112 of DNA by the polymerase chain reac- tion . See Polymerase chain reaction of gene copy ...
... selective agent , 1.5 for maintaining expression plasmids , 17.16 Amplification , 1.3 of bacteriophage › plaques on nitrocellu- lose filters , 2.112 of DNA by the polymerase chain reac- tion . See Polymerase chain reaction of gene copy ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml