Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 97
Page 18-7
... sequence of the protein of interest is known or can be deduced from the corresponding nucleic acid sequence , it is possible to raise specific antisera by immunizing animals with short synthetic peptides ( for review , see Lerner 1984 ) ...
... sequence of the protein of interest is known or can be deduced from the corresponding nucleic acid sequence , it is possible to raise specific antisera by immunizing animals with short synthetic peptides ( for review , see Lerner 1984 ) ...
Page 1-12
... sequence analysis ; Maxam and Gilbert chemical degradation of DNA method for DNA sequence analy- sis ; Nucleotide sequence data banks spectrophotometric determination of the amount , E.5 topoisomerase I , 5.89 , 15.36 , 15.50 ...
... sequence analysis ; Maxam and Gilbert chemical degradation of DNA method for DNA sequence analy- sis ; Nucleotide sequence data banks spectrophotometric determination of the amount , E.5 topoisomerase I , 5.89 , 15.36 , 15.50 ...
Page 1-13
... sequence analysis , 13.7-13.9 , 13.65-13.70 Taq , reactions and uses , 5.34 , 5.50-5.51 DNA sequence analysis , 13.7-13.9 polymerase chain reaction , 14.2–14.4 , 14.16 . See also Polymerase chain reaction terminal transferase ( terminal ...
... sequence analysis , 13.7-13.9 , 13.65-13.70 Taq , reactions and uses , 5.34 , 5.50-5.51 DNA sequence analysis , 13.7-13.9 polymerase chain reaction , 14.2–14.4 , 14.16 . See also Polymerase chain reaction terminal transferase ( terminal ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml