Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xviii
... SINGLE AGAROSE GEL TO TWO NITROCELLULOSE FILTERS 9.41 Transfer of DNA from Agarose Gels to Nylon Membranes 9.42 ... Single - stranded Probes 10.18 PRODUCTION OF SINGLE - STRANDED DNA PROBES USING BACTERIOPHAGE M13 VECTORS 10.19 Primer ...
... SINGLE AGAROSE GEL TO TWO NITROCELLULOSE FILTERS 9.41 Transfer of DNA from Agarose Gels to Nylon Membranes 9.42 ... Single - stranded Probes 10.18 PRODUCTION OF SINGLE - STRANDED DNA PROBES USING BACTERIOPHAGE M13 VECTORS 10.19 Primer ...
Page xxv
... Single - stranded Target DNA 15.53 Design and Selection of Mutagenic Oligonucleotides 15.54 Hybridization of Oligonucleotides to the Template DNA and Primer Extension 15.58 Transfection of E. coli and Screening for Mutants 15.59 ...
... Single - stranded Target DNA 15.53 Design and Selection of Mutagenic Oligonucleotides 15.54 Hybridization of Oligonucleotides to the Template DNA and Primer Extension 15.58 Transfection of E. coli and Screening for Mutants 15.59 ...
Page E-30
... Single - stranded nucleic acids bind less tightly to hy- droxyapatite than do double - stranded nucleic acids and are eluted at lower phosphate concentrations . Chromatography on hydroxyapatite can therefore be used to separate single ...
... Single - stranded nucleic acids bind less tightly to hy- droxyapatite than do double - stranded nucleic acids and are eluted at lower phosphate concentrations . Chromatography on hydroxyapatite can therefore be used to separate single ...
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acetate acrylamide Adjust the volume agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography cloning column containing deionized H₂O Dissolve dithiothreitol DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract final concentration fragment gene H H H H OH hybridization Incubate intensifying screen lb/sq ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl NH3 H nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF precipitation prepared protein radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution strain supernatant syringe target protein Telephone termini transfected transfer Tris Cl pH vectors wash western blotting µg/ml