Molecular Cloning: A Laboratory Manual, Book 3 |
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Page xviii
... Double - stranded DNA Probes 10.6 NICK TRANSLATION OF DNA 10.6 Stock Solutions Used in Nick Translation 10.7 Protocol for Nick Translation 10.8 Alternative Protocol for Nick Translation 10.11 SYNTHESIS OF UNIFORMLY LABELED DNA PROBES ...
... Double - stranded DNA Probes 10.6 NICK TRANSLATION OF DNA 10.6 Stock Solutions Used in Nick Translation 10.7 Protocol for Nick Translation 10.8 Alternative Protocol for Nick Translation 10.11 SYNTHESIS OF UNIFORMLY LABELED DNA PROBES ...
Page xxv
... STRANDS THAT CONTAIN URACIL ( KUNKEL METHOD ) 15.74 Preparation of Uracil - containing Single - stranded DNA 15.76 SOLVING PROBLEMS THAT ARISE IN OLIGONUCLEOTIDE - MEDIATED , SITE - DIRECTED MUTAGENESIS 15.80 Using Mutagenesis to Study ...
... STRANDS THAT CONTAIN URACIL ( KUNKEL METHOD ) 15.74 Preparation of Uracil - containing Single - stranded DNA 15.76 SOLVING PROBLEMS THAT ARISE IN OLIGONUCLEOTIDE - MEDIATED , SITE - DIRECTED MUTAGENESIS 15.80 Using Mutagenesis to Study ...
Page E-30
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SEPARATION OF SINGLE - STRANDED AND DOUBLE - STRANDED DNA BY HYDROXYAPATITE CHROMATOGRAPHY Nucleic acids bind to ... SINGLE-STRANDED AND DOUBLE-STRANDED DNA HYDROXYAPATITE CHROMATOGRAPHY E ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. SEPARATION OF SINGLE - STRANDED AND DOUBLE - STRANDED DNA BY HYDROXYAPATITE CHROMATOGRAPHY Nucleic acids bind to ... SINGLE-STRANDED AND DOUBLE-STRANDED DNA HYDROXYAPATITE CHROMATOGRAPHY E ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml