Molecular Cloning: A Laboratory Manual, Book 3 |
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Page A-2
... solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 g of KH , PO , and 12.54 g of K2HPO , in 90 ml of deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with ...
... solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 g of KH , PO , and 12.54 g of K2HPO , in 90 ml of deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ml with ...
Page B-9
A Laboratory Manual Joseph Sambrook, Tom Maniatis. STOCK SOLUTIONS TABLE B.7 Preparation of Commonly Used Stock Solutions Solution Method of preparation Comments TABLE B.7 ( continued ) Solution 2.5 M CaCl2 Method. 30 % Acrylamide 40 ...
A Laboratory Manual Joseph Sambrook, Tom Maniatis. STOCK SOLUTIONS TABLE B.7 Preparation of Commonly Used Stock Solutions Solution Method of preparation Comments TABLE B.7 ( continued ) Solution 2.5 M CaCl2 Method. 30 % Acrylamide 40 ...
Page E-8
... solution , add 0.2 volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution ...
... solution , add 0.2 volume of fresh 5 % hypophosphorous acid and 0.12 volume of fresh 0.5 M sodium nitrite . Mix carefully . Important : Check that the pH of the solution is < 3.0 . Hypophosphorous acid is usually supplied as a 50 % solution ...
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Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml