Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 21
Page A-2
... sterile solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 g of KH , PO , and 12.54 g of K2HPO , in 90 ml of deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ...
... sterile solution of 0.17 м KH2PO4 , 0.72 м K2HPO4 . ( This solution is made by dissolving 2.31 g of KH , PO , and 12.54 g of K2HPO , in 90 ml of deionized H2O . After the salts have dissolved , adjust the volume of the solution to 100 ...
Page A-5
... sterile glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle ) . Vortex the culture to ensure that the glycerol is evenly dispersed . Transfer the culture to a labeled storage tube equipped with a ...
... sterile glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle ) . Vortex the culture to ensure that the glycerol is evenly dispersed . Transfer the culture to a labeled storage tube equipped with a ...
Page A-7
... sterile 20 % maltose solution for every 100 ml of medium . Make up a sterile 20 % stock solution of maltose as follows : maltose H2O to 100 ml 20 g Sterilize the solution by filtration through a 0.22 - micron filter . Store the sterile ...
... sterile 20 % maltose solution for every 100 ml of medium . Make up a sterile 20 % stock solution of maltose as follows : maltose H2O to 100 ml 20 g Sterilize the solution by filtration through a 0.22 - micron filter . Store the sterile ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml