Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 2
... 24 g glycerol 4 ml Shake until the solutes have dissolved and sterilize by
autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . Allow the solution to
cool to 60°C or less , and then add 100 ml of a sterile solution of 0 . 17 M KH ,
PO4 , 0 .
... 24 g glycerol 4 ml Shake until the solutes have dissolved and sterilize by
autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle . Allow the solution to
cool to 60°C or less , and then add 100 ml of a sterile solution of 0 . 17 M KH ,
PO4 , 0 .
Page 5
To store bacteria , pick a single , well - isolated colony with a sterile inoculating
needle and stab the needle several times through the agar to ... 15 ml of sterile
glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle
) .
To store bacteria , pick a single , well - isolated colony with a sterile inoculating
needle and stab the needle several times through the agar to ... 15 ml of sterile
glycerol ( sterilized by autoclaving for 20 minutes at 15 lb / sq . in . on liquid cycle
) .
Page 7
Add 1 ml of a sterile 20 % maltose solution for every 100 ml of medium . Make up
a sterile 20 % stock solution of maltose as follows : maltose H , 0 to 100 ml
Sterilize the solution by filtration through a 0 . 22 - micron filter . Store the sterile ...
Add 1 ml of a sterile 20 % maltose solution for every 100 ml of medium . Make up
a sterile 20 % stock solution of maltose as follows : maltose H , 0 to 100 ml
Sterilize the solution by filtration through a 0 . 22 - micron filter . Store the sterile ...
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User Review - iayork - LibraryThingthe BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review
Contents
BOOK | 3 |
Identification and Analysis of Recombinants 2 108 | 30 |
TRANSFECTION MEDIATED BY DEAEDEXTRAN 16 | 41 |
Copyright | |
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Common terms and phrases
acetate activity added Adjust aliquots allow amount antibody antigen Appendix appropriate approximately aqueous assay bacterial bacteriophage base binding buffer carried cells centrifugation Chapter chromatography Cl pH cloned coli column complete concentration containing culture deionized water described desired detected dilution Dissolve EDTA efficiency electrophoresis enzyme et al ethanol expression extract Figure filter final fragment gene growth hybridization Incubate levels ligation mammalian cells medium method mg/ml microfuge tube minutes mixture mRNAs nucleic acid pellet phase phosphate Place plasmid plate polymerase precipitation prepared procedure Products promoter protein radioactive radiolabeled reaction remove restriction room temperature sample screen selection sequence sodium solution specific step sterile stored strain TABLE Telephone termini tion transcription transfection transfer transformation Tris ug/ml usually vectors volume wash western blotting ОН
Bibliographic information
| Title | Molecular Cloning: A Laboratory Manual, Book 3 Molecular Cloning: A Laboratory Manual, Joseph Sambrook, ISBN 0879693096, 9780879693091 |
| Authors | Joseph Sambrook, E. F. Fritsch, Tom Maniatis |
| Edition | 2 |
| Publisher | Cold Spring Harbor Laboratory, 1989 |
| Original from | the University of Michigan |
| Digitized | 23 Feb 2010 |
| ISBN | 0879693096, 9780879693091 |
| Length | 412 pages |
| Export Citation | BiBTeX EndNote RefMan |