Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 18-10
... stored in small aliquots that are labeled with the name of the animal and the date of the bleed . Polyclonal antisera are best stored undiluted , whereas monoclonal antibodies should be stored at concentrations of > 1 mg / ml in ...
... stored in small aliquots that are labeled with the name of the animal and the date of the bleed . Polyclonal antisera are best stored undiluted , whereas monoclonal antibodies should be stored at concentrations of > 1 mg / ml in ...
Page 18-55
... stored indefinitely in water in a sealed plastic bag without any diminution in the intensity of staining . However , fixed polyacrylamide gels stored in water will swell and may distort during storage . To avoid this problem , store ...
... stored indefinitely in water in a sealed plastic bag without any diminution in the intensity of staining . However , fixed polyacrylamide gels stored in water will swell and may distort during storage . To avoid this problem , store ...
Page A-7
... stored indefinitely at room temperature . A 2 % gelatin solution is made by adding 2 g of gelatin to a total volume of 100 ml of H2O and autoclaving the solution for 15 minutes at 15 lb / sq . in . on liquid cycle . TM Per liter : · 1 м ...
... stored indefinitely at room temperature . A 2 % gelatin solution is made by adding 2 g of gelatin to a total volume of 100 ml of H2O and autoclaving the solution for 15 minutes at 15 lb / sq . in . on liquid cycle . TM Per liter : · 1 м ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml