Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 14
Page 1-27
... target sequence Mercaptoethanol . See ẞ - Mercaptoethanol MES ( 2- [ N - morpholino ] ethanesulfonic acid ) pKa and buffering range , B.1 stock solution , 1.78 Methotrexate , use in dihydrofolate reduc- tase amplification system , 16.28 ...
... target sequence Mercaptoethanol . See ẞ - Mercaptoethanol MES ( 2- [ N - morpholino ] ethanesulfonic acid ) pKa and buffering range , B.1 stock solution , 1.78 Methotrexate , use in dihydrofolate reduc- tase amplification system , 16.28 ...
Page 1-35
... target sequence , 14.12- 14.13 methods , 14.18-14.19 mixed oligonucleotide primed amplifica- tion of cDNA , 14.7 precautions to prevent contamination , 14.14 quantitation of concentration of target sequences , 14.30-14.33 reaction ...
... target sequence , 14.12- 14.13 methods , 14.18-14.19 mixed oligonucleotide primed amplifica- tion of cDNA , 14.7 precautions to prevent contamination , 14.14 quantitation of concentration of target sequences , 14.30-14.33 reaction ...
Page 1-44
... target sequence empirical determination for oligonucle- otide hybridized to target se- quence , 11.55-11.57 estimating , 9.50-9.51 , 11.46-11.49 for mutagenesis , 15.55 irreversible , 11.48-11.49 Tn10 , transposon encoding resistance ...
... target sequence empirical determination for oligonucle- otide hybridized to target se- quence , 11.55-11.57 estimating , 9.50-9.51 , 11.46-11.49 for mutagenesis , 15.55 irreversible , 11.48-11.49 Tn10 , transposon encoding resistance ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amino acids amount antibody antigen aqueous phase assay autoclaving autoradiographic bacteriophage T4 DNA blunt-ended bromophenol blue cDNA centrifugation chromatography codon coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency eluted Escherichia coli ethanol ethidium bromide expression extract H H OH Incubate intensifying screen ligation linkers lysate mammalian cells medium method mg/ml microfuge tube mixture Molecular mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pasteur pipette pellet phenol phosphate pipette plasmid PMSF polyclonal precipitation prepared promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample Saran Wrap SDS gel-loading buffer SDS-polyacrylamide gel sequence single-stranded sodium sterile stock solution stored strain supernatant syringe target protein Telephone termini transcription transfected transfer Tris Cl pH vectors wash western blotting µg/ml